Cdc20 associates with the kinase aurora2/Aik

The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3.

The MLK (mixed lineage) ser/thr kinases are most closely related to the MAP kinase kinase kinase family. In addition to a kinase domain, MLK1, MLK2 and MLK3 each contain an SH3 domain, a leucine zipper domain and a potential Rac/Cdc42 GTPase-binding (CRIB) motif. The C-terminal regions of the proteins are essentially unrelated. Using yeast two-hybrid analysis and in vitro dot-blots, we show tha...

Yeast Pak1 kinase associates with and activates Snf1.

Members of the Snf1/AMP-activated protein kinase family are activated under conditions of nutrient stress by a distinct upstream kinase. Here we present evidence that the yeast Pak1 kinase functions as a Snf1-activating kinase. Pak1 associates with the Snf1 kinase in vivo, and the association is greatly enhanced under glucose-limiting conditions when Snf1 is active. Snf1 kinase complexes isolat...

Phosphatidylinositol 3-Kinase Activation Syk Associates with Clathrin and Mediates

Casein kinase 2 associates with and phosphorylates dishevelled.

The dishevelled (dsh) gene of Drosophila melanogaster encodes a phosphoprotein whose phosphorylation state is elevated by Wingless stimulation, suggesting that the phosphorylation of Dsh and the kinase(s) responsible for this phosphorylation are integral parts of the Wg signaling pathway. We found that immunoprecipitated Dsh protein from embryos and from cells in tissue culture is associated wi...

Doing the CDC20 shuffle

or perhaps a decade, Suc1 was the hardy little protein that featured in almost every cell cycle paper. But it was used only as a reagent—its binding to cyclindependent kinases (such as Cdc2 or Cdc28) yielded nearly pure MPF. Then the budding yeast version, called Cks1, was found to be essential for growth. Now, May Morris, Steven Reed, and colleagues (The Scripps Research Institute, La Jolla, C...